News |  Sitemap |  Contact
PDF Export  | 

Registration of TEM images with TrakEM and bUnwarpJ

Abstract:

I work on reconstructing the first optic lobe in Drosophila, lamina, using TEM images of Drosophila brain serial sections.
In order to register my image stacks I use the ImageJ plugin bUnwarpJ in a serial mode to obtain a global registration, needed for the reconstruction.
I, also, use TrakEM to create registered montages of images. I create and align the montages at the same time.
A major problem is the folds in the serial sections, resulting in black lines crossing my TEM images. Hence, a simple affine transformation is not sufficient to register my images. However, the problem can be solved by automatic detection of landmarks and then applying the bUnwarpJ elastic registration.


Keywords:
Segmentation, registration, 3D viewer, reconstruction, Drosophila, neuroscience.

Author
Marta Rivera-Alba and Gonzalo G. de Polavieja

Organisation   
Universidad Autonoma de Madrid

Homepage   

Short Biography   
I have BA in Theoretical Physics and a Master in Biophysics, both of them by the Universidad Autonoma de Madrid. Currently I am doing my PhD in the anatomy of Drosophila first optic neuropil, lamina, under the supervision of G. G. de Polavieja in the Universidad Autonoma de Madrid. I am specially interested in checking the optimization theories of brain anatomy in Drosophila, and in the predictions that I can obtain from that.

© Luxembourg Institute of Science and Technology | Legal Notice