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Image analysis for evaluating the efficiency of some natural antifouling agents for the patina inhibition on monumental fountains


All exposed cultural assets made mainly by stone are susceptible to biodeterioration processes. Monuments, statues and artistic fountains are frequently covered by biological patinas. The use of toxic biocides and mechanical removal are the most common methods for their control. The using of natural antifouling agents are tentatively used as new control methods in order to inhibit the formation of biofilms on the surface of stone materials. Five of these natural inhibitors (zosteric acid, capsaicin, cinnamaldehyde, poly-alkylpyridinium salts and Ceramium botryocarpum extract) and a biocide (algophase) were tested on Carrara marble samples immersed in an open air simulating artificial fountain. The natural antifoulants agents’ efficiency was evaluated at different time intervals analyzing the marble samples with confocal and epifluorescence microscopy. The percentage of inhibition of each antifouling product has been calculated on the pictures taken with these two types of microscopes using the ImageJ program.

antifoulants agents, natural inhibitors, phototrophic biofilm, monumental fountain, image analysis, ImageJ

Oana-Adriana Cuzman



Short Biography
I am 30 years old and I came from Romania. I studied in Romania, in my home town, Iassy. I graduated from the Faculty of Biology in 2000. I awarded the master degree in Environmental Management at the Faculty of Industrial Chemistry in 2003. Since 2005 I have worked as a biologist in the National Institute of Conservation and Restoration, Bucharest, Romania. In September 2006 I started the PhD program within the European project EPISCON, developing my research on the phototrophic biofilms dwelled on exposed monumental stone, at ICVBC-CNR, Florence, Italy.

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