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Automatic Measurements of Cell Structures: Programing and Applications

Full / Half Time Slot: Half Time Slot (90 min)

Contact / Speaker Name

Dimiter Prodanov

Workshop Title

Automatic Measurements of Cell Structures: Programing and Applications

Abstract

This workshop demonstrates several imaging techniques in fuorescence microscopy together with programing
techniques in writing ImageJ plugins.
Techniques are applied in tasks of automatic identifcation and measurement of (i) synaptic boutons in cultured brain cells and of (ii) axonal profiles loaded with fuorescent tracer infrozen nerve sections. In the first part, mathematical granulometry is used to isolate potential synaptic boutons,
which are then measured automatically. Using this approach, quantitative effects of different treatments of cell
cultures can be measured. In the second part, channel subtraction and granulometry are used to reconstruct
the relationship of myelinated fibers dimensions from a specic population with their tracer content. To relate
the area occupied by the tracer within an axonal prole to the area of the corresponding fiber (axon + myelin
sheath) paired sets of measurements were performed on representative images. A linear regression relationship
could be established for the equivalent diameter of the fiber and the equivalent diameter of the axonal signal.
This allows to reconstruct the total population of labeled fibers solely from the uorescence measurements.

Participant Requirements

basic knowledge of (fluoresecence) microscopy; basic knowledge of Java

Biography of Speaker

Education:
2006 - PhD degree, Twente University, Enschede, Netherlands
1999 - MD degre, Medical University of Sofia, Sofia, Bulgaria

Experience:
2006 - Neural Engineering Rehabilitation Laboratory / Catholic University of Louvain, Brussels, Belgium

2002 - 2005 Leiden University Medical Center, Leiden and Twente University, Enschede, Netherlands

2000 - 2002 USMeds Bulgaria Inc

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