Single molecule imaging is now widely performed with advanced microscopy. If one have good microscopy such
as total internal refection microscopy (TIRFM) with ultra-sensitive detectors, and if ones prepare the sample
properly, he/she can acquire single molecule imaging data easily. However, the analysis of single molecule imaging data have great difficulties for pure biologist because automatic particle detection and tracking is essentially
required. Here, I introduce particle tracking and analysis ImageJ plugin, named PTA. This plugin can perform
automatic particle detection and tracking with nanometer accuracy by 2-dimensional Gaussian fitting implemented with Java Native Interface (JNI), which enables us stress-free, fast particle detection. The tracked particles data can be shown as x-y trace and trajectories, fluorescence time trajectories, mean square displacement (MSD) and velocity with JFreeChart based graphics.
Particle tracking, 2-dimensional Gaussian fitting, Java Native Interface
I am an assistant professor in Osaka University, Japan. My research interest is to develop novel techniques like optical microscopy system and image processing tools in order to elucidate the biological system such as cellular dynamics.
Presenting author: Yoshiyuki Arai
Organisation: The Institute of Scientific and Industrial Research, Osaka University