The number of fluorescent sensor and reporter molecules and their use in intact cells has significantly increased in past years. Yet, the analysis of data from single cells or cell population is usually a very time-consuming enterprise. Here we introduce FluoQ, a new macro for ImageJ, that analyses multiparameter time-lapse microscopy data with minimal user input. FluoQ provides statistical analysis of all measured parameter and outputs the results in multiple graphic and numeric displays. It also analyses libraries of experiments automatically. We demonstrate the usefulness by applying FluoQ to data analysis in the optimization of a novel FRET reporter for monitoring the calcium/calmodulin-binding inositol trisphosphate kinase A (ITPKA) performance in intact cells.
Presenting author: Frank Stein
Organisation: Cell Biology and Biophysics Unit, EMBL, Meyerhofstraße 1, 69117 Heidelberg, Germany.
co-authors: Manuel Kress and Carsten Schultz